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Secondary Antibodies
Anti-biotin, HRP-linked Antibody
Anti-biotin, HRP-linked Antibody from Cell Signaling Technology
(1)
Write a review
Citations:
(143)
Supplier Page
Supplier Page from
Cell Signaling Technology for
Anti-biotin, HRP-linked Antibody
Description
This antibody may cross react with endogenously expressed biotinylated proteins. Careful titration may be needed
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-biotin, HRP-linked Antibody
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
7075S
Quantity
1 ml
Host
Goat
Conjugate/Tag
biotin
Research Area
Related Products
Target
Biotin (for Secondary Abs)
Applications
W
Reactivity
All
Target Species
All
Add to Compare List
Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (143)
(1)
BioE3 identifies specific substrates of ubiquitin E3 ligases
Nature Communications
November 23, 2023
Orhi Barroso-Gomila, Laura Merino-Cacho, Veronica Muratore, Coralia Perez, Vincenzo Taibi, Elena Maspero, Mikel Azkargorta, Ibon Iloro, Fredrik Trulsson, Alfred C. O. Vertegaal,
et al.
at RT. Primary antibodies used as follows:
Cell Signaling Technology
: anti-biotin-HRP (1/1000; Cat#
7075
S), anti-alpha-Actinin (1/5000; Cat#
6487S
), anti-
PCM1
(1/1000; Cat#
5213S
);
SinoBiological
: anti-BirA
(2)
Prolyl hydroxylase 2 inhibits glycolytic activity in colorectal cancer via the NF‑κB signaling pathway
International Journal of Oncology
November 15, 2023
Lisha Xiang, Hao Wei, Wentao Ye, Shuang Wu, Ganfeng Xie
Inc.), -
mouse
(
1:5,000
; cat. no.
7076
;
Cell Signaling Technology
, Inc.), or -
goat
(
1:5,000
; cat. no.
7075
;
Cell Signaling Technology
, Inc.) secondary antibodies were used for
incubation
for 1 h at room temperature.
Electrophoretic mobility shift assay
(3)
Garcinia mangostana L. Pericarp Extract and Its Active Compound α-Mangostin as Potential Inhibitors of Immune Checkpoint Programmed Death Ligand-1
Molecules
October 9, 2023
Sandar Naing, Nichawadee Sandech, Arnatchai Maiuthed, Sumet Chongruchiroj, Jaturong Pratuangdejkul, Pattamapan Lomarat
peroxidase (HRP)-conjugated anti-rabbit IgG (#
7074
,
Cell Signaling Technology
, MA, USA) and anti-biotin (#
7075
,
Cell Signaling Technology
, MA, USA) at
1:2000
dilution. The protein bands were exposed using enhanced
(4)
Click chemistry-based thiol redox proteomics reveals significant cysteine reduction induced by chronic ethanol consumption
Redox Biology
August 1, 2023
Peter S. Harris, Courtney D. McGinnis, Cole R. Michel, John O. Marentette, Richard Reisdorph, James R. Roede, Kristofer S. Fritz
signal from specific antibodies. The anti-biotin antibody was purchased from
Cell Signaling Technology
(
7075
) and was used at
1:1000
dilution. Blots were visualized using a
Bio-Rad
ChemiDoc MP Imaging System
and
(5)
GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail
Cell death & disease
June 28, 2023
Zou X, Ma L, Zhang Y, Zhang Q, Xu C, Zhang D, Chu Y, Zhang J, Li M, Zhang H,
et al
.
mouse
anti-
Snail
(
sc-271977
,
Santa Cruz
), rabbit anti-p66β (
ab76925
,
Abcam
),
anti-biotin, HRP-linked
(
7075
,
Cell Signaling Technology
), and rabbit anti-IgG (
2729
,
Cell Signaling Technology
),
mouse
anti-α-tubulin
(6)
GATS tag system is compatible with biotin labelling methods for protein analysis
Scientific Reports
June 23, 2023
Kohdai Yamada, Fumiya Soga, Soh Tokunaga, Hikaru Nagaoka, Tatsuhiko Ozawa, Hiroyuki Kishi, Eizo Takashima, Tatsuya Sawasaki
laboratory), α-tubulin (
MBL
,
PM054-7
,
1:5000
), GST (M071-7,
1:5000
), and biotin (
Cell Signaling Technology
, #
7075
,
1:1000
). The following primary antibodies were used in this study: IκBα (CST L35A5,
1:1000
), RelA (CST
(7)
RBBP4 is an epigenetic barrier for the induced transition of pluripotent stem cells into totipotent 2C-like cells
Nucleic Acids Research
June 23, 2023
Wangfang Ping, Yingliang Sheng, Gongcheng Hu, Hongxin Zhong, Yaoyi Li, YanJiang Liu, Wei Luo, Chenghong Yan, Yulin Wen, Xinxiu Wang,
et al.
biotinylation
of RBBP4 and
RBBP7
was detected with anti-Biotin antibody (
Cell Signaling Technology
,
7075
).
Trophoblast
derivation from
mESCs
Cell culture plates were pre-coated with 0.3% gelatin.
Cdx2
-tdTomato
(8)
Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening system
Communications Biology
May 9, 2023
Akira Nozawa, Ryoko Miyazaki, Yoshinao Aoki, Reina Hirose, Ryosuke Hori, Chihiro Muramatsu, Yukinori Shigematsu, Keiichirou Nemoto, Yoshinori Hasegawa, Keiko Fujita,
et al.
with
horse
radish
peroxidase-conjugated anti-biotin antibody (
Cell Signaling Technology
, Danvers, MA, #
7075
,
1:5000
) or anti-FLAG antibody (
Sigma Aldrich
Japan,
A8592
,
1:5000
). The synthesised proteins were detected
(9)
Mitochondrial calcium uniporter activates TFEB-driven autophagy to promote migration of breast cancer cells
Iran J Basic Med Sci
April 4, 2023
Lin Yuan, Qimou Lin, Fei Shen, Yong Li, Junda Li, Bo Xu
cat.
7074
, Beverly, USA) and
anti-biotin, HRP-linked
antibody (
1:2000
;
Cell Signaling Technology
, cat.
7075
, Beverly, USA) for 1 hr at room temperature. The
membranes
were then visualized using the enhanced chemiluminescence
(10)
Diverse yeast antiviral systems prevent lethal pathogenesis caused by the L-A mycovirus
Proceedings of the National Academy of Sciences
March 14, 2023
Sabrina Chau, Jie Gao, Annette J. Diao, Shi Bo Cao, Amirahmad Azhieh, Alan R. Davidson, Marc D. Meneghini
at 4°C overnight and probed with
1:3,000
horseradish peroxidase (HRP)-conjugated
horse
anti-
mouse
(
7075
;
Cell Signaling Technology
) or
goat
anti-rabbit (
7074
;
Cell Signaling Technology
) secondary antibody.
(11)
P-Rex1 is a novel substrate of the E3 ubiquitin ligase Malin associated with Lafora disease
Neurobiology of Disease
February 1, 2023
L. Kumarasinghe, M.A. Garcia-Gimeno, J. Ramirez, U. Mayor, J.L. Zugaza, P. Sanz
rabbit anti-GLUT1 (
PA1–46152
.
Invitrogen
. Madrid, Spain), goat anti-biotin-HRP-conjugated antibody (#
7075
.
Cell Signaling Technology
, Barcelona, Spain), and
mouse
anti-Na+/K+-ATPase
(
ab7671
.
Abcam
. Madrid,
(12)
CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis
Scientific Reports
December 1, 2022
Shusei Sugiyama, Kohdai Yamada, Miwako Denda, Satoshi Yamanaka, Satoshi Ozawa, Ryo Morishita, Tatsuya Sawasaki
AGIA (produced in our laboratory)41, α-tubulin (
MBL
,
PM054-7
), and biotin (
Cell Signaling Technology
, #
7075
). To detect the proteins spotted on the array,
anti-DDDDK-tag mAb-Alexa Fluor 647
(
MBL
,
M185-A64
) was
(13)
Processes in DNA-damage response from a whole-cell multi-omics perspective
iScience
November 18, 2022
James C. Pino, Alexander L.R. Lubbock, Leonard A. Harris, Danielle B. Gutierrez, Melissa A. Farrow, Nicole Muszynski, Tina Tsui, Stacy D. Sherrod, Jeremy L. Norris, John A. McLean,
et al.
Signaling),
PLK1
(
ab17056
,
Abcam
), or phosphorylated
PLK1
(
ab133442
,
Abcam
). Binding of an anti-
mouse
(
7075
,
Cell Signaling
) or an anti-rabbit (
7074
,
Cell Signaling
) HRP-conjugated secondary antibody was detected
(14)
Caveolae couple mechanical stress to integrin recycling and activation
eLife
October 20, 2022
Lolo FN, Pavón DM, Grande A, Elósegui Artola A, Segatori VI, Sánchez S, Trepat X, Roca-Cusachs P, Del Pozo MA.
Alexa Fluor–488 phalloidin
(
Thermo Fisher Scientific
); HRP-linked anti-biotin from
Cell Signaling
(#
7075
); and
Alexa Fluor–647 phalloidin
(
Thermo Fisher Scientific
). EZ-Link SulfoNHS-SS-biotin was from Thermo
(15)
Aberrant Cholesterol Metabolism and Wnt/β-Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer Growth
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
October 1, 2022
Zheng S, Lin J, Pang Z, Zhang H, Wang Y, Ma L, Zhang H, Zhang X, Chen M, Zhang X,
et al
.
Ki‐67 (8D5) Mouse mAb
,
Cell Signaling Technology
,#
9499
"
Anti‐biotin, HRP‐linked
Antibody",
Cell Signaling Technology
,#
7075
Anti‐Frizzled 5
Rb Polyclonal Antibody,
Sigma‐Aldrich
,
06‐756
Wnt3a (C64F2) Rabbit mAb
,
Cell Signaling Technology
(16)
In vitro sepsis induces Nociceptin/Orphanin FQ receptor (NOP) expression in primary human vascular endothelial but not smooth muscle cells
PLOS ONE
September 15, 2022
Mark F. Bird, Barbara Gallacher-Horley, John McDonald, David G. McVey, Fatin Al-Janabi, Remo Guerrini, Girolamo Calo, Shu Ye, Jonathan P. Thompson, David G. Lambert
HRP-conjugated secondary antibodies (1hr room temperature;
1:1000
dilution 5% milk/TBS-T solution; #
7075
,
Cell Signalling
Technology). Chemiluminescence detection, using the
ChemiDoc™ MP imaging system
(
Bio-Rad
,
(17)
Non-Apoptotic Caspase-3 Activation Mediates Early Synaptic Dysfunction of Indirect Pathway Neurons in the Parkinsonian Striatum
International Journal of Molecular Sciences
May 13, 2022
Tim Fieblinger, Chang Li, Elena Espa, M. Angela Cenci
were incubated with HRP-linked secondary antibodies (
anti-biotin HRP-linked
antibody
Cell Signaling
, #
7075
;
anti-rabbit-IgG HRP-linked
antibody,
Cell Signaling
, #
7074
) and bands were visualized by chemiluminescence
(18)
Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83
Frontiers in Physiology
April 27, 2022
Yue Gao, Nan Chen, Xiangle Zhang, Emma Y. Li, Wei Luo, Jie Zhang, Wenqiang Zhang, Sheng Li, Jian Wang, Suning Liu
by chemiluminescence. The primary antibodies used in this study were HRP-linked
goat
anti-biotin (#
7075
,
Cell Signaling Technology
, CST), mouse anti-FLAG (#
F1804
,
Sigma-Aldrich
), rabbit anti-V5 (#
ab9116
,
(19)
Integrin mediates cell entry of the SARS-CoV-2 virus independent of cellular receptor ACE2
The Journal of biological chemistry
March 1, 2022
Liu J, Lu F, Chen Y, Plow E, Qin J.
antibody (Novagen #70796), and biotinylated proteins were detected by biotin antibody (
Cell signaling
#
7075
). SPR experiments SPR experiments were carried out on the BIAcore T200 (Cytiva, former GE Healthcare)
(20)
Differential maturation and chaperone dependence of the paralogous protein kinases DYRK1A and DYRK1B
Scientific reports
February 14, 2022
Papenfuss M, Lützow S, Wilms G, Babendreyer A, Flaßhoff M, Kunick C, Becker W.
polyclonal
anti-mouse IgG (H&L
, #
610-703-124
,
Rockland
, Gilbertsville, PA, USA),
goat
anti-biotin IgG (#
7075
,
Cell Signaling Technology
, Danvers, MA, USA). Plasmids The NEBuilder Assembly Tool v2.2.7 and the
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Reviews
Write a Review
(1)
5/27/2020
HRP-Conjugated Anti-Biotin Antibody Working Well for WB
Wei Xu
I used this antibody to detected biotin signals by western blot and it worked well.
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Anti-biotin, HRP-linked Antibody from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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